Doctor-Developed · Peer-Reviewed · Evidence-Tiered · Reviewed August 2026
PeptideMath — the peptide dosing reference
Credentialed, doctor-developed, and built on data — published trials and approved labels, not what your Facebook group or a Reddit thread swears by. Every dose on this site carries a grade telling you the strength of the evidence behind it. No other peptide reference does that.
Written and reviewed by doctorate-level clinicians.✓ Last medically reviewed August 8, 2026 · Every dose graded by evidence tier · No products sold, ever.
50+ compounds & blendsExact syringe mathHonest evidence gradesResearch use only
Dose calculator
Pick a compound (or tap one from the index below), enter your vial's actual label mass and diluent volume — the math is exact. Reference doses are informational, not endorsements.
The microdosing approach: start at the smallest dose with any reported effect, hold it, and only titrate up if nothing happens. These are the conventions circulating in the community as of August 2026.
No clinical data exists for any microdosing protocol below
Not one of these schedules has been tested in a controlled human trial. Every number is anecdote — self-reported by users and repeated by clinics selling the service. The claimed benefits are hypothetical; the risks of the parent compounds are real and documented. Use entirely at your own risk. If you have a medical condition or take medication, this is a conversation for your physician, not a website.
SemaglutideGLP-1 · Tier A drug, Tier D as a microdose
0.25–2.4 mg/wk
0.05–0.25 mg/wk
Appetite "quieting," anti-inflammatory and longevity effects without the weight loss or GI side effects.
TirzepatideGIP/GLP-1 · Tier A drug, Tier D as a microdose
2.5–15 mg/wk
1–2.5 mg/wk
Same logic — roughly 1/5 of a typical dose, popularized by longevity influencers.
BPC-157Tier C at any dose
250–500 mcg/day
100–250 mcg/day
"Maintenance" tissue support at the lowest dose users report feeling anything from.
IpamorelinGHRP · Tier C
100–300 mcg, 1–3×/day
50–100 mcg nightly
A single small nightly pulse for sleep/recovery without desensitization or appetite effects.
Melanotan IITier C; priapism/mole risk at any dose
250–500 mcg/day loading
50–100 mcg/day
Gradual pigmentation with less nausea — the most common reason MT-II users microdose.
NAD+Coenzyme, not a peptide · Tier C
50–100 mg/injection
25–50 mg/injection
Working up slowly because injection-site pain and flushing are dose-limiting.
The MED playbook (if you're going to do this anyway)
Start at the bottom of the convention range — you can always go up; you can't un-take a dose.
Hold each dose ≥ 2–4 weeks before judging it. Most reported effects are slow.
Change one variable at a time. One compound, one dose. Stacks make attribution impossible.
Track something objective — weight, sleep data, labs — not just how you feel.
Small doses still need accurate draws: a 0.1 mg semaglutide dose measured on a U-100 syringe leaves zero room for sloppy math. Use the calculator.
What "no data" actually means here
No trial has tested whether GLP-1 microdoses in healthy people produce any benefit — the longevity claims extrapolate from animal studies and full-dose patient data.
No one knows the dose floor: the dose small enough to skip side effects but large enough to do anything may not exist.
Class risks don't scale down linearly — thyroid C-cell warnings and pancreatitis signals for GLP-1s apply to the compound, not just the full dose.
Tier C compounds microdosed are still Tier C: anecdote layered on anecdote.
Every common compound
Tap any card to load it into the calculator with sensible defaults. Search or filter by category.
Reconstitution, step by step
Lyophilized (freeze-dried) powder + bacteriostatic water. Same procedure for every vial; only the numbers change.
Gather: vial, bacteriostatic water (0.9% benzyl alcohol), alcohol swabs, one larger mixing syringe (1–3 mL), insulin syringes for dosing.Sterile water works but has no preservative — single-use only. Bacteriostatic water is the multi-dose standard.
Swab both stoppers — the peptide vial and the water vial — with an alcohol wipe. Let them air-dry.
Draw your chosen volume of water (use the calculator above — it suggests a volume that puts your dose on an easy-to-read syringe mark).
Inject the water slowly down the inside wall of the peptide vial. Do not jet it directly onto the powder.A hard stream can foam and shear fragile peptides. Tilt the vial and let it run down the glass.
Swirl gently. Never shake. Most peptides dissolve clear within a few minutes. If it stays cloudy or has particles, don't inject it.
Label the vial — compound, concentration (mg/mL), and reconstitution date — and refrigerate at 2–8 °C.
Subcutaneous injection basics
Technique
Needle: insulin syringe, 29–31 gauge, 5/16" (8 mm) is the common choice.
Sites: abdomen (≥ 2 inches from the navel), front/outer thigh, back of the arm, flank. Rotate sites every injection.
Angle: pinch a fold of skin; insert at 45–90° depending on how much subcutaneous fat you have.
Inject slowly, withdraw, apply light pressure. No rubbing.
One needle, one use. Needles dull instantly; reuse hurts more and raises infection risk. Sharps container for disposal.
Hard rules
GLP-1 class (semaglutide, tirzepatide, etc.): titration is mandatory. Starting at maintenance doses causes severe GI effects. Start at the lowest step, hold ≥ 4 weeks per step.
Never inject a solution that is cloudy, discolored, or has particulates.
Never share vials or syringes with anyone.
Site pain, spreading redness, warmth, or fever after injection → that's an infection question, not a forum question. Seek care.
If a compound's card here says Tier C or D, understand what that means: no controlled human dosing data exists.
Storage & handling
Lyophilized (unmixed) vials
Cool, dark, dry. Refrigeration (2–8 °C) is good practice; freezing (−20 °C) for long holds.
Keep away from light and heat — peptides degrade with both.
Sealed lyophilized peptides are generally stable for months to years when kept cold. Heat during shipping is the bigger threat for most compounds.
Reconstituted vials
Refrigerate at 2–8 °C, always. Never freeze a mixed vial.
With bacteriostatic water, the standard multi-dose convention is use within ~28 days. Stability varies by peptide; some degrade faster.
With plain sterile water: no preservative — treat as single-session use.
Minimize warm time on the counter. Draw your dose, put it back.
Evidence tiers — what the dose number is actually built on
The reconstitution arithmetic is identical regardless of tier. The confidence in the dose itself is not.
A
Approved drug or robust human RCTs establishing dosing. The number is real.
B
Some human clinical data — trials, foreign approval, or a discontinued program. Dosing is informed but not settled for the enhancement use.
C
Animal studies plus self-reported anecdote only. No controlled human dosing exists. Treat the number as a folklore convention.
D
Minimal or purely theoretical. Even animal dosing is thin; product identity/purity is often unverifiable.
Sourcing red flags — how gray-market product fails you
No third-party certificate of analysis (HPLC + mass spec) tied to your batch number — purity claims without testing are marketing.
Underfilled vials: independent testing routinely finds vials containing less — sometimes far less — than the labeled mass. Your dose math is only as good as the label.
Pre-mixed "ready to inject" product shipped warm — no cold chain, no preservative disclosure.
"For human use" claims from a research-chemical vendor — that's a vendor ignoring the law, which tells you how they treat quality too.
Independent testing services exist that will assay a vial you send in. For anything you inject repeatedly, that's cheap insurance.
Peptide dosing — frequently asked questions
The questions every first-time user asks, answered without the forum noise.
How do I calculate how many units of a peptide to draw?
Three numbers: vial mass (mg on the label), water added (mL), and your dose. Concentration = vial mass ÷ water. Volume to draw = dose ÷ concentration. On a U-100 insulin syringe, units = mL × 100. Example: 5 mg in 2 mL = 2.5 mg/mL; a 250 mcg dose = 0.1 mL = 10 units. The calculator at the top does this instantly.
How much bacteriostatic water should I add to a peptide vial?
Any amount — it changes concentration, not the dose. More water = bigger, easier-to-measure draws; less = smaller draws from the same vial. A practical target is a volume that puts your dose between 10 and 30 units on a U-100 syringe. The calculator pre-fills a suggested volume for every compound.
What's the difference between bacteriostatic water and sterile water?
Bacteriostatic water contains 0.9% benzyl alcohol, which suppresses bacterial growth and makes a vial usable as a multi-dose container. Plain sterile water has no preservative — treat anything mixed with it as single-session use.
How long does a reconstituted peptide vial last in the fridge?
The standard multi-dose convention with bacteriostatic water is about 28 days at 2–8 °C. Stability varies by peptide — some degrade faster — and plain sterile water shortens this dramatically. If your dose schedule outlasts the window, the calculator flags it and suggests mixing a partial vial.
How many mg is 10 units on an insulin syringe?
It depends entirely on your concentration — units measure volume, not drug mass. 10 units on a U-100 syringe is 0.1 mL. At 2.5 mg/mL that's 250 mcg; at 10 mg/mL it's 1 mg. This is why copying someone else's "unit" numbers without knowing their reconstitution is the most common dosing mistake.
What is peptide microdosing?
Taking a fraction of the conventional dose — often 1/5 to 1/10 — aiming for benefits with fewer side effects, most visibly with GLP-1s like semaglutide and tirzepatide. Understand clearly: no clinical trial has validated any microdosing protocol. Our microdosing section lists the circulating conventions and labels them as exactly what they are: anecdote.
Are research peptides legal to buy?
Most gray-market peptides are sold as "research use only" chemicals — not FDA-approved for human use, and vendors selling them for injection operate in a legal gray zone that regulators periodically enforce against. Several compounds here (semaglutide, tirzepatide, tesamorelin, PT-141) are approved prescription drugs available legitimately through a physician. This site is informational and doesn't sell anything; nothing here is legal advice.
Are the doses on this site medically recommended?
No — they're documented, which is different. Tier A doses come from approved labels and human trials. Tier C and D numbers are community conventions with no controlled human data, and we say so on every card. That honesty is the entire point of the evidence-tier system.
About PeptideMath
PeptideMath exists because the dosing information people actually use comes from screenshots, forum threads, and vendors with something to sell.
We're doctorate-level clinicians who kept watching the same thing happen: intelligent, motivated people making milligram-to-unit conversion errors, copying someone else's syringe marks without knowing their concentration, and treating forum folklore as if it were trial data. The compounds people are injecting deserve at least the arithmetic being right.
So we built the reference we wished existed: exact reconstitution math, every common compound in one place, and — the part nobody else does — an honest grade on every dose number telling you whether it comes from an FDA-approved label, a human trial, or pure anecdote. Where no data exists, we say "no data exists." We don't sell peptides, we don't take vendor money, and we don't pretend certainty we don't have.
Use it well, be skeptical of everything — including us — and talk to your own physician about anything you put in your body.
The PeptideMath clinical team Medically reviewed August 8, 2026 · How we grade evidence
Reconstitution math: concentration = vial mass ÷ diluent volume. Volume to draw = dose ÷ concentration. Units = volume (mL) × (100 for U-100, 40 for U-40). Doses per vial = vial mass ÷ dose. Syringe convention: a U-100 syringe reads 100 units per 1 mL; "units" are syringe graduations, not International Units of any hormone. Blends: component ratios are vendor-defined; the per-dose breakdown assumes the label ratio is accurate.
This site computes numbers you supply and summarizes published evidence as of August 2026. It does not sterility-test product, verify label accuracy, or replace a physician, pharmacist, or IRB-approved protocol. Compounds marked investigational have no approved human dose. Research use only.